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MMP Substrate III, Fluorogenic-Calbiochem

品牌
Sigma-Aldrich
货号
444256
规格纯度
A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination.
参考价格
3539.04 *本价格含增值税费
促销
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数量
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产品名称:
MMP Substrate III, Fluorogenic-Calbiochem
DABCYL-GABA-PQGL-E(EDANS)-AK-NH2, TNO211
产品介绍:

产品说明

一般描述

A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity [kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, and 21,000 M-1s-1 for MMP-1; at 37°C, pH 7.6 ]. Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Also useful for MMP activity measurements in synovial fluid and culture medium.
A fluorescence energy transfer (FRET) peptide substrate with MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity (kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, 21,000 M-1s-1 for MMP-1; pH = 7.6 at 37°C). Exhibits a high degree of sensitivity, which is not affected by optical disturbances in biological media. Excitation max.: ~340 nm; emission max.: ~485 nm.

包装

500 μg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Primary Target
Detection of MMP activity
Product does not compete with ATP.
Reversible: no
Cell permeable: no

警告

Toxicity: Standard Handling (A)

序列

DABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂

外形

Supplied as a trifluoroacetate salt.

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

其他说明

Beekman, B., et al. 1996. FEBS Lett390, 221.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式C63H88N16O14S
分子量1325.54

产品性质

质量水平100
测定≥97% (HPLC)
形式lyophilized solid
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
protect from light
颜色 red
溶解性DMSO: soluble
Tris-buffered saline, pH 7.6: soluble
荧光λex ~340  nm
λem ~485 nm
运输ambient
储存温度−20℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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