MystiCq^® microRNA qPCR Assay Primer

产品说明

一般描述

The MystiCq^® microRNA qPCR Assay Primer is an integral part of the MystiCq microRNA qPCR Assay System. It has been designed to target specific microRNAs with the MystiCq Universal PCR primer and the MystiCq^® microRNA SYBR^® Green qPCR ReadyMix^™ on cDNA templates generated by the MystiCq microRNA cDNA Synthesis Mix kit.

生化/生理作用

The microRNA hsa-miR-194-5p, also known as hsa-miR-194-2, acts as a tumor-suppressor in osteosarcoma. It targets cadherin-2 (CDH2). It has been shown to suppress the invasion, growth and metastasis in various tumor models. miR-194 inhibits cell proliferation in hepatocellular carcinoma and acts as a prognostic marker.

特点和优势

• Proprietary design features of assay primers allow for specificity towards mature microRNAs vs. precursor-microRNAs
• Extensive design and testing of oligo-dT adapter primer to incorporate complementary Universal PCR Primer sequence
• No self-complementarity or primer dimer artifacts with the Universal PCR Primer
• Optimized primer Tm designed to match the Universal PCR Primer
• Universal cycling conditions ensures robust amplification for all assays in profiling experiments
• Optimized PCR product Tm (75-78° C)

其他说明

Formerly hsa-miR-194. Compatible with mmu-miR-194 and rno-miR-194.

法律信息

MystiCq is a registered trademark of Qiagen Beverly Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

基本信息

产品性质

安全信息