PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20)

产品说明

一般描述

Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.

免疫原

KLH conjugated to phosphotyrosine, glycine, and alanine

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

ELISA (see comments)

Immunoblotting (1:500-1:1000, see application references)

Frozen Sections (see application references)

Immunocytochemistry (see application references)

Immunoprecipitation (see comments)

警告

Toxicity: Standard Handling (A)

外形

In 10 mM sodium phosphate, pH 7.4.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
Any cell lysate or tissue extract

其他说明

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息