PhosphoDetect Anti-RhoA (pSer¹⁸⁸) Rabbit pAb

产品说明

一般描述

This PhosphoDetect Anti-RhoA (pSer¹⁸⁸) Rabbit pAb is validated for use in Immunoblotting, ELISA for the detection of RhoA (pSer¹⁸⁸).
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~22 kDa RhoA protein when expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.
Recognizes the ~22 kDa RhoA protein, expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.

免疫原

a synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH
Human

包装

100 μL in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (1:1000)
ELISA (1:2000)

警告

Toxicity: Standard Handling (A)

外形

In PBS, 1 mg/ml BSA, 50% glycerol.

重悬

Do not aliquot.

分析说明

Positive Control
RhoA GST fusion protein

其他说明

Ellerbroek, S.M., et al. 2003. J. Biol. Chem.278, 19023.
Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
Dong, J.M., et al. 1998. J. Biol. Chem.273, 22554.
Lang, P., et al. 1996. EMBO J.15, 510.
Does not recognize unphosphorylated RhoA. The immunogen sequence is conserved in rat and mouse RhoA, but cross-reactivity has not been tested. The immunogen sequence is not found in the RhoB and RhoC sequences. For immunoblotting, the membrane was incubated with antibody diluted in PBS/5% nonfat milk/0.04% Tween^® 20 detergent for 1 h at room temperature. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

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