产品说明
一般描述
Recombinant, human thioredoxin expressed in E. coli. A ubiquitous protein with two redox-active half-cysteine residues that function as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman′s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T-cells and human PBL blasts. Occurs as a single polypeptide chain.
Recombinant, human thioredoxin expressed in E. coli. A ubiquitous protein with two redox-active half-cysteine residues. Functions as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman’s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T cells and human PBL.
包装
100 μg in Plastic ampoule
警告
Toxicity: Standard Handling (A)
单位定义
One unit (insulin reduction unit) is calculated by measuring the change in absorbance at 650 nm with 330 µM DTT as the reductant at 25°C, pH 6.5.
重悬
Reconstitute in physiological buffer containing 3 mM EDTA and 1-5 mM DTT.
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
其他说明
Noda, N., et al. 1997. Nippon Rinsho 55, 461.
Wollman, E.E., et al. 1997. Biochem. Biophys. Res. Commun. 230, 602.
Masutani, H., et al. 1996. Immunol. Lett. 54, 67.
Saito, I., et al. 1996. Arthritis Rheumatism, 39, 773.
Saito, N., et al. 1995. J. Immunol. 154, 3194.
Buchanon, B.B., et al. 1994. Arch. Biochem. Biophys. 314, 257.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
MDL编号 | MFCD00132423 |
产品性质
质量水平 | 100 |
重组 | expressed in E. coli |
测定 | ≥90% (SDS-PAGE) |
形式 | lyophilized solid |
specific activity | ≥1.7 units/mg protein |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze |
运输 | ambient |
储存温度 | −20℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich