HAR, G human cell line

产品说明

一般描述

Homozygous: No
Consanguineous: No

细胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA图谱分析

HLA DRB1: 0301 07
HLA DRB3: 101
HLA DRB4: 101
HLA DQAP: 2.1 4.1
HLA DQA1: 0501 0201
HLA DQB1: 201
HLA DPB1: 0401 0402

血清学分析

HLA A: 1 32
HLA C: 7
HLA B: 8 14
Bw4/bw6: 6

培养基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

传代培养常规

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他说明

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

产品性质