Tumor Necrosis Factor-α, human (hTNF-α)

产品名称：

产品介绍：

产品说明

一般描述

Tumor necrosis factor a (TNF-a) is a multifunctional cytokine, majorly produced by activated macrophages and is involved in the pathogenesis of many acute and chronic inflammatory condition.

Recombinant, Tumor Necrosis Factor-α, human (hTNF-α), is produced in Pichia pastoris and purified by standard chromatographic techniques.

Primary structure: One polypeptide chain (157 amino acids), identical to natural hTNF-a.

特异性

Human hTNF-α is effective on human and mouse cells.
Species specificity: human, mouse

应用

Tumor Necrosis Factor-α (hTNF-α)

Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.
Has activating and growth stimulating activities on a variety of normal cells.
Has antiviral activity on many cell types in vitro.
Can cause tumor necrosis of certain tumors in vivo.

生化/生理作用

Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.

质量

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

序列

Chain Length 157 AA
One polypeptide chain (157 amino acids), identical to natural hTNF-α.

单位定义

EC₅₀ definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).

外形

Solution, filtered through 0.2 μm pore size membrane.

制备说明

Working concentration: 1 ng/ml
For complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.
Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.
Storage conditions (working solution): -15 to -25 ℃
It is recommended to store the solution in aliquots at -15 to -25 ℃.
Note: Avoid repeated freezing and thawing.

其他说明

For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng

产品性质

质量水平	100
生物来源	human
重组	expressed in yeast
测定	≥95% (SDS-PAGE)
形式	solution
效能	≤0.01 ng/mL EC₅₀
specific activity	>1*10^8 U/mg (>1 x 10^⊥8 U/mg, <0.01 ng/ml (hTNF-a, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.)
分子量	17,000 Da
包装	pkg of 1,000,000 U (10 μg, 1ml)
manufacturer/tradename	Roche
储存条件	avoid repeated freeze/thaw cycles
杂质	<0.1 EU/μgtested (LAL test)
UniProt登记号	Q9UBM5
运输	dry ice
储存温度	−20℃
Gene Information	human ... TNF(7124)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	does not flash
闪点(C)	does not flash