Anti-Neurofilament L Antibody

产品说明

一般描述

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10 nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.

特异性

Recognizes Neurofilament-Light (NF-L)

免疫原

Purified porcine NF-L

应用

Detect Neurofilament L using this Anti-Neurofilament L Antibody validated for use in IC, IH, IH(P) & WB.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers
Western Blot Analysis:
1:1000 dilution of a previous lot detected NEUROFILAMENT L on 10 μg of mouse brain lysates.

Immunocytochemistry:
A 1:200-1:500 dilution of a previous lot was used in IC.

Optimal working dilutions must be determined by the end user.

质量

Immunohistochemistry(paraffin) Analysis:
Neurofilament-L (cat. # AB9568) staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:800, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber-like- staining (brown) around a Purkinje cells.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Human Cerebellum

目标描述

68-70 kDa

联系

Replaces: 04-1112

外形

Unpurified
Rabbit serum. Contains no preservative.

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.

分析说明

Control
Brain tissue

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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