产品说明
应用
5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.
包装
50 μL in poly bottle
基本信息
| 经验(实验)分子式 | C42H54N5O21P3 |
| 分子量 | 1057.82 |
| PubChem化学物质编号 | 329769180 |
| NACRES | NA.22 |
产品性质
| 质量水平 | 200 |
| 测定 | >95% (HPLC) |
| 形式 | solution |
| reaction suitability | reaction type: click chemistry |
| 溶解性 | Tris buffer: 100 mM (pH 7.5) |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| SMILES string | O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
Sigma-Aldrich
m.cnreagent.com
