产品说明
一般描述
This antisense RNA probe (human β-actin) is in vitro transcribed in the presence of digoxigenin (DIG)-UTP. The transcript has a length of 588 bases. 550 bases are complementary to the 5′ region of human β-actin mRNA (nucleotides 69 to 6l8, EMBL: HSAC07). The additional 25 bases at the 5′ end, and the 13 additional bases at the 3′ end of the transcript are specific for the promoter/polylinker region of the transcription vector.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
应用
Actin RNA Probe, DIG-labeled, is specifically useful for evaluating the quality and quantity of various RNA species and can be used:
组分
Actin RNA Probe, DIG-labeled, is supplied in autoclaved, DEPC-treated water.
序列
Agarose gel electrophoresis under denaturing conditions and subsequent northern blot analysis reveal a defined band of 588 bases.
制备说明
Working solution: Dilute Sample
其他说明
仅用于生命科学研究。不可用于诊断。
产品性质
形式 | solution |
质量水平 | 100 |
包装 | pkg of 2 μg |
manufacturer/tradename | Roche |
环保替代产品特性 | Designing Safer Chemicals Learn more about the Principles of Green Chemistry. |
环保替代产品分类 | Aligned |
运输 | dry ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | No data available |
闪点(C) | No data available |
Sigma-Aldrich