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Anti-Calcitonin Gene Related Protein Antibody

品牌
Sigma-Aldrich
货号
AB5705
规格纯度
Chemicon®, from chicken
参考价格
6526.19 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Calcitonin Gene Related Protein Antibody
CGRP
产品介绍:

产品说明

特异性

CGRP (Calcitonin Gene Related Peptide).

免疫原

A 14 amino acid synthetic peptide from rat CGRP I (processed active peptide). The immunogen sequence is identical at 13 of 14 residues between rat/mouse CGRP I and CGRP II. {KDNFVPTNVGSEAF-NH2}

应用

Immunohistochemistry: 1:500 See Protocol below.

Optimal working dilutions must be determined by the end user.



AB5705 has been used successfully on perfusion fixed tissue. Perfusion: 1) Ca2+-free Tyrode′s solution followed by 2) a paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5705. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Research Category
Neuroscience
Research Sub Category
CNS Control of Metabolism

Hormones & Receptors
This Anti-Calcitonin Gene Related Protein Antibody is validated for use in IH for the detection of Calcitonin Gene Related Protein.

外形

Purified chicken IgY. Liquid containing 0.05% sodium azide.
Format: Purified

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源chicken
质量水平100
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆polyclonal
species reactivityrat
manufacturer/tradenameChemicon®
technique(s)immunohistochemistry: suitable
NCBI登记号NM_001033952.1
NM_001033953.1
NM_001741.2
UniProt登记号P01258
运输dry ice
Gene Informationhuman ... CALCA(796)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

Sigma-Aldrich

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