Anti-DFF40 Antibody

产品说明

特异性

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (Enari et al. 1998; Sakahira et al. 1998). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN and DFF40 and human CAD, respectively (Halenbeck et al. 1998; Liu et al. 1998; Mukae et al. 1998). DFF45/ICAD is the inhibitory protein of DFF40/CAD (Enari et al. 1998; Sakahira et al. 1998; Liu et al. 1997) and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually cuases the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

免疫原

A peptide corresponding to amino acids 147-164 of mouse CAD (Enari et al. 1998). The sequence differs from human DFF40 by two amino acids (Halenbeck et al. 1998; Liu et al. 1998).

应用

Western blot: 1:500-1:1,000

K562 or Jurkat whole cell lysate can be used as a positive control and a 40 kDa band should be detected.

Optimal working dilutions must be determined by end user.
Detect DFF40 using this Anti-DFF40 Antibody validated for use in WB.

外形

Format: Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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