Anti-Fluorescein

产品说明

一般描述

Monoclonal antibody to fluorescein.
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.

Contents
Lyophilizate, stabilized

特异性

The monoclonal antibody reacts with free and bound fluorescein.

应用

Use Anti-Fluorescein for the detection of fluorescein-labeled compounds using:

• ELISA
• Immunohistocytochemistry
• In situ hybridization
• Western blot

Anti-Fluorescein has been used to capture amplicon in quadruple-tagging PCR and nucleic acid lateral flow assay for simultaneous detection of foodborne pathogens.

制备说明

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:

• ELISA: 2 to 4 μg/ml
• Immunohistocytochemistry: 0.5 to 2 μg/ml
• In situ hybridization: 0.2 to 0.4 μg/ml
• Western blot: 0.5 to 2 μg/ml

Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.

重悬

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Reconstitute for 15 minutes.

其他说明

仅用于生命科学研究。不可用于诊断。

产品性质

质量水平	100
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	B13-DE1, monoclonal
形式	lyophilized
包装	pkg of 100 μg
manufacturer/tradename	Roche
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

象形图
警示用语：	Warning
危险声明	H317 - H412
预防措施声明	P261 - P273 - P280 - P333 + P313 - P362 + P364 - P501
危险分类	Aquatic Chronic 3 - Skin Sens. 1
储存分类代码	11 - Combustible Solids
WGK	WGK 2
闪点(F)	does not flash
闪点(C)	does not flash