Anti-Histone H3, Acetylated (1-20) Rabbit pAb

产品说明

一般描述

Anti-Histone H3, Acetylated (1-20), rabbit polyclonal, recognizes the ~17 kDa acetylated histone H3 protein. It is validated for use in Western blotting, immunocytochemistry & immunoprecipitation.
Recognizes the ~17 kDa acetylated histone H3 protein.
Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa acetylated histone H3 protein.

免疫原

Human
a diacetylated synthetic peptide [ARTKQTAR_AcKSTGG_AcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH

包装

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (10-500 ng/ml, chemiluminescence)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (see comments)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
HeLa cells

其他说明

Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349.
Braunstein, M., et al. 1993. Genes Devel.7, 592.
Perry, C.A., et al. 1993. Biochemistry32, 13605.
Lin, R., et al. 1989. J. Cell. Biol.108, 1577.
For immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser¹⁰ on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息