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位置:首页 > 品牌 > Sigma-Aldrich > Anti-Hsp70 Mouse mAb (C92F3A-5)

Anti-Hsp70 Mouse mAb (C92F3A-5)

品牌
Sigma-Aldrich
货号
386032
规格纯度
liquid, clone C92F3A-5, Calbiochem®
参考价格
5854.74 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Hsp70 Mouse mAb (C92F3A-5)
Anti-Heat Shock Protein 70
产品介绍:

产品说明

一般描述

Mouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.
Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections).
Recognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.

免疫原

Hsp70 from HeLa cells
Human

包装

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

ELISA (see comments)

Flow Cytometry (see comments)

Frozen Sections (5 µg/ml)

Immunoblotting (1 µg/ml)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (see comments)

Paraffin Sections (5 µg/ml)

警告

Toxicity: Standard Handling (A)

外形

In PBS, 50% glycerol, pH 7.2.

重悬

Following iniital thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
L929 cells, Human colon cancer tissue

其他说明

Does not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Hang, H., and Fox, M.H. 1995. Cytometry19, 119.
Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589.
Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724.
Gower, D.J., et al. 1989. J. Neurosurg.70, 605.
Milarski, K., et al. 1989.J. Cell Biol.108, 413.
Vass, K., et al. 1988. Acta Neuropathologica77, 128.
Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆C92F3A-5, monoclonal
形式liquid
包含≤0.1% sodium azide as preservative
species reactivity (predicted by homology)all
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型IgG1
运输wet ice
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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