Anti-Hsp70 Mouse mAb (C92F3A-5)

产品说明

一般描述

Mouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.
Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections).
Recognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.

免疫原

Hsp70 from HeLa cells
Human

包装

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

ELISA (see comments)

Flow Cytometry (see comments)

Frozen Sections (5 µg/ml)

Immunoblotting (1 µg/ml)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (see comments)

Paraffin Sections (5 µg/ml)

警告

Toxicity: Standard Handling (A)

外形

In PBS, 50% glycerol, pH 7.2.

重悬

Following iniital thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
L929 cells, Human colon cancer tissue

其他说明

Does not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Hang, H., and Fox, M.H. 1995. Cytometry19, 119.
Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589.
Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724.
Gower, D.J., et al. 1989. J. Neurosurg.70, 605.
Milarski, K., et al. 1989.J. Cell Biol.108, 413.
Vass, K., et al. 1988. Acta Neuropathologica77, 128.
Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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安全信息