产品说明
特异性
Recognizes Huntingtin protein phosphorylated at serine 421.Minimal reactivity is expected with the non-phosphorylated form of the protein.
免疫原
Epitope: phosphoSer 421
Synthetic peptide from human Huntingtin protein. It is expected that the antibody may cross react with rat and mouse due to conservation of the immunogen sequence (8/9).
应用
Western blotting: 1:500-1:3,000 using ECL. Reacts with the ~190 kD Huntingtin protein in unstimulated human PC-3 whole cell lysate. Additional bands at ~130 may also be detected.
ELISA: 1:10,000-1:40,000
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Detect Huntingtin using this Anti-Huntingtin Antibody, phosphoSer 421 validated for use in WB.
Research Sub Category
Neurodegenerative Diseases
外形
Affinity purified immunoglobulin. Liquid in 0.02M Potassium phosphate, 0.15M Sodium Chloride with 0.01% sodium azide.
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | western blot: suitable |
NCBI登记号 | NM_002111.6 |
UniProt登记号 | P42858 |
运输 | dry ice |
Gene Information | human ... HTT(3064), SLC6A4(6532) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich