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Anti-MYCBP2 Antibody, clone PM-8A9

品牌
Sigma-Aldrich
货号
MABN2397
规格纯度
clone PM-8A9, from mouse
参考价格
5016.79 *本价格含增值税费
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数量
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产品名称:
Anti-MYCBP2 Antibody, clone PM-8A9
E3 ubiquitin-protein ligase MYCBP2, Myc-binding protein 2, Pam/highwire/rpm-1 protein, Protein associated with Myc
产品介绍:

产品说明

一般描述

E3 ubiquitin-protein ligase MYCBP2 (UniProt: O75592; also known as Myc-binding protein 2, Pam/highwire/rpm-1 protein, Protein associated with Myc, MYCBP2) is encoded by the MYCBP2 (also known as KIAA0916, PAM) gene (Gene ID: 23077) in human.MYCBP2 protein mediates the ubiquitination and subsequent proteasomal degradation of TSC2/tuberin. It is ubiquitously expressed, but brain and thymus display exceptionally abundant expression. It is shown to co-localize with TSC1 and TSC2 along the neurites and in the growth cones. MYCBP2 may also function as a facilitator or regulator of transcriptional activation by MYC and have a role during synaptogenesis. It is reported to negatively regulate neuronal growth, synaptogenesis, and synaptic strength. Mice with MYCBP2-deficiency in peripheral sensory neurons show prolonged thermal hyperalgesia. Loss of MYCBP2 is also linked to constitutively activated p38 MAPK and increased expression of several proteins involved in receptor trafficking. Down-regulation of MYCBP2 in the spinal cord of adult rats is shown to enhance their. MYCBP2 is also shown to be an inhibitor of adenylyl cyclase. (Ref.: Holland, S et al. (2011). J. Biol. Chem. 286(5): 3671-3680).

特异性

Clone PM-8A9 specifically detects MYCBP2 in HEK293 cells. It targets an epitope within the first 150 amino acids in the N-terminal region.

免疫原

MBP (Maltose-binding protein)-tagged recombinat fragment of 150 amino acids from the N-terminal region of human MYCBP2.

Epitope: N-terminus

应用

Research Category
Neuroscience
Anti-MYCBP2 Antibody, clone PM-8A9, Cat. No. MABN2397, is a highly specific mouse monoclonal antibody that targets MYCBP2 (PAM) and has been tested in Immunoprecipitation and Western blotting.
Immunoprecipitation Analysis: A representative lot detected MYCBP2 (Courtesy of Roberta L. Beauchamp, Massachusetts General Hospital, Boston, USA).

Western Blotting Analysis: A representative lot detected endogenous PAM (MYCBP2)and overexpressed Myc-tagged PAM (Courtesy of Roberta L. Beauchamp, Massachusetts General Hospital, Boston, USA).

质量

Evaluated by Western Blotting in HEK293 cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MYCBP2 in 10 µg of HEK293 cell lysate.

目标描述

~460 kDa observed; 510.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆PM-8A9, monoclonal
species reactivityhuman
technique(s)immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_055872
UniProt登记号O75592
运输ambient
Gene Informationhuman ... MYCBP2(23077)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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