产品说明
一般描述
Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain.
特异性
This antibody detects a 65 kDa protein corresponding to the molecular weight of N-WASP on SDS-PAGE immunoblots of neonatal rat brain lysate. It is also detects 65 kDa proteins in A431, human endothelial, and SKN-SH cells. It does not recognize the 63 kDa WASP protein in Jurkat cell lysate.
免疫原
Synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminus of human N-WASP. This N-WASP peptide sequence is 100% homologous to rat and mouse N-WASP, and has low homology to the corresponding region in the human WASP sequence.
应用
Anti-N-WASP Antibody is an antibody against N-WASP for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
Western blot: 1:1000 ELISA: 1:2000
外形
Affinity-purified antibody is supplied in 100 μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
储存及稳定性
Maintain at -20°C for up to one year from date of receipt. Do not aliquot.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | rat, human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NM_003941.2 |
UniProt登记号 | O00401 |
运输 | wet ice |
Gene Information | human ... WASL(8976) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | not combustible |
闪点(C) | not combustible |
Sigma-Aldrich