产品说明
特异性
Recognizes NAP-22 [Neuronal acidic protein 22, CAP-23, BASP1].
免疫原
Recombinant full length rat NAP-22.
Epitope: Complete sequence
应用
Research Sub Category
Developmental Neuroscience
Neuroregenerative Medicine
Research Category
Neuroscience
Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 55 kDa NAP-22 protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 10%. Overnight incubation with the antibody is recommended.
Optimal working dilutions must be determined by the end user.
This Anti-NAP-22 Antibody, complete sequence is validated for use in WB for the detection of NAP-22.
外形
Semi-purified immunoglobulin. Liquid in TBS containing 0.05% sodium azide.
Format: Purified
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
分析说明
Control
POSITIVE CONTROL: Neonatal rat brain extract. NAP-22 is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze NAP-22 content by immunoblot.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | rat |
manufacturer/tradename | Chemicon® |
technique(s) | western blot: suitable |
NCBI登记号 | NM_006317.3 |
UniProt登记号 | P80723 |
运输 | dry ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
Sigma-Aldrich