Anti-Neurofilament H (200 kDa) Antibody, CT

产品说明

一般描述

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

特异性

Strong and specific staining for NF-H with no cross-reactivity for NF-M or NF-L. Reacts with both the phosphorylated and dephosphorylated forms of neurofilament 200 kDa. Stains neurons of the central and peripheral nervous system, including new processes in all organs. Preferentially stains nerve processes.

免疫原

Recombinant fusion protein containing the extreme C-terminus of rat NF-H, prepared from E. coli inclusion bodies by dissolution in urea followed by DEAE-cellulose ion exchange chromatography. Immunogen does not include the lysine-serine-proline
Epitope: C-terminus

应用

Immunoblotting

Immunohistochemistry: 1:200

Optimal working dilutions must be determined by end user.
Detect Neurofilament H (200 kDa) using this Anti-Neurofilament H (200 kDa) Antibody, C-terminus validated for use in IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

目标描述

200 kDa

联系

Replaces: AB1982

外形

Unpurified
Neat rabbit antisera, no preservatives.

储存及稳定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Brain, Peripheral Nerve

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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