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Anti-Nkx-5.1 Antibody

品牌
Sigma-Aldrich
货号
AB5744
规格纯度
Chemicon®, from rabbit
参考价格
6022.61 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-Nkx-5.1 Antibody
NK-2
产品介绍:

产品说明

特异性

Recognizes Nkx5.1, (NK-2 family), a homeodomain transcription factor.

免疫原

Synthetic peptide, amino acids 10-23 from mouse Nkx5.1.

应用

Research Category
Neuroscience
Western blot: 1:200-1:1,000 using ECL.

Immunohistochemistry: 1:200-1:1,000.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Developmental Neuroscience

Neuronal & Glial Markers
This Anti-Nkx-5.1 Antibody is validated for use in WB, IH for the detection of Nkx-5.1.

外形

Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

储存及稳定性

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

生物来源rabbit
质量水平100
抗体形式saturated ammonium sulfate (SAS) precipitated
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, human
manufacturer/tradenameChemicon®
technique(s)immunohistochemistry: suitable
western blot: suitable
NCBI登记号NM_001105574.1
UniProt登记号A8MU06
运输dry ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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