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Anti-Oct-4 Antibody

品牌
Sigma-Aldrich
货号
AB3209
规格纯度
Chemicon®, from rabbit
参考价格
6022.61 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Oct-4 Antibody
POU5F1
产品介绍:

产品说明

一般描述

Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

特异性

Recognizes Oct4 (POU5F1). The molecular weight of the protein is 38,601 Daltons.

免疫原

Synthetic peptide, amino acids 282-297of Oct4.

应用

Research Sub Category
Pluripotent & Early Differentiation
This Anti-Oct-4 Antibody is validated for use in IC, IH, WB for the detection of 41186.
Research Category
Stem Cell Research
Western blot: 1:200-1:1,000 using ECL. Oct-4 in cells typically shows ~40-44kDa band or doublet.

Immunohistochemistry: 1:200-1:1,000.

Immunocytochemistry: 1:200-1:1,000.

Optimal working dilutions must be determined by the end user.

目标描述

44 kDa

外形

Affinity Purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
ImmunoAffinity Purified

储存及稳定性

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

分析说明

Control
Pluripotent human embryonic stem (ES) cells

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity purified immunoglobulin
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman, mouse
manufacturer/tradenameChemicon®
technique(s)immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
inputsample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
NCBI登记号NM_002701.4
NM_203289.3
UniProt登记号Q01860
运输wet ice
Gene Informationhuman ... POU5F1(5460)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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