Anti-pan-MAGUK Antibody, clone K28/86

产品说明

一般描述

The MAGUKs are membrane-associated guanylate kinase related (MAGUK) proteins that are defined by their inclusion of PDZ, SH3 and GUK domains. The GUK domain that they have is structurally very similar to that of the guanylate kinases, which converts ATP into ADP. However, it is known to be catalytically inactive as the P-Loop which normally binds ATP is absent. It is thought that the MAGUKs have adopted the GUK domain for their own purposes, primarily based on its ability to form protein-protein interactions with cytoskeleton proteins, microtubule/actin based machinery and molecules involved in signal transduction. In addition many of the MAGUK proteins also contain regions homologous of CaMKII, WW and L27 domains. Some of the members of the MAGUK family of proteins include DLG4 (Disks large homolog 4), DLG2, PSD95, MPP1 and other cytoskeleton-interacting proteins.

特异性

This antibody exhibits cross reactivity against recombinant Chapsyn-110, SAP97, and SAP102. (UC Davis/NIH NeuroMab Facility, http://neuromab.ucdavis.edu)

免疫原

Recombinant protein corresponding to a fusion domain of human PSD95/SAP90 proteins.

应用

Anti-pan-MAGUK Antibody, clone K28/86 is an antibody against pan-MAGUK for use in IH, WB & IP.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected the MAGUK family of proteins in rat cerebellum and rat hypothalamus tissues.

Immunoprecipitation Analysis: A previous lot of this antibody was successfully used by an independent laboratory in IP. (Rasband, M., et al. (2002). The Journal of Cell Biology. 159(4):663–672.)
Research Sub Category
Signaling Neuroscience
Research Category
Neuroscience

质量

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected members of the MAGUK protein family on 10 µg of rat brain tissue lysate.

目标描述

~ 85-110 kDa observed

外形

Format: Purified
Protein G
Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Rat brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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