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Anti-Septin-3 Antibody, clone 5C1.2

品牌
Sigma-Aldrich
货号
MABN1530
规格纯度
clone 5C1.2, from mouse
参考价格
5352.52 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Septin-3 Antibody, clone 5C1.2
Neuronal-specific septin-3E
产品介绍:

产品说明

一般描述

Neuronal-specific septin-3 (UniProt Q9UH03) is encoded by the SEPT3 (also known as SEP3) gene (Gene ID 55964) in human. Septins are GTPases capable of forming higher-order oligomers and filamentous polymers that contact with cell membrane and cytoskeleton. Septins are involved in various cellular processes, including cytokinesis, karyokinesis, exocytosis, and cell shape maintenance. Septins exist in complexes that are composed of ordered non-polar arrays of multiple different septins. Human genome contains 13 septin genes that are grouped into the SEPT2 subgroup (septins 1, 2, 4, and 5), the SEPT3 subgroup (septins 3, 9, and 12), the SEPT6 subgroup (septins 6, 8, 10, 11, and 14), and the SEPT7 subgroup (septin 7). All subgroups contain the well conserved GTP-binding domain and differ mainly in their C-terminal region, where coiled-coil sequences are found in SEPT2, SEPT6, and SEPT7, but not SEPT3, subgroups. Septin-3 is the only septin to be expressed almost exclusively in brain tissue, where it is enriched in nerve terminals, suggesting a role in synaptic vesicle function. Increased septin-3 expression is reported in several brain tumours and SEPT3 polymorphisms are linked to Alzheimer′s disease. PKG-1 is shown to phosphorylate septin-3 in vitro, non-phosphorylated septin-3 localizes to the peripheral membrane, whereas the phosphorylated form is found in the cytosol. Murine and rat septin-3 is reported to form complexes with septin-5 and septin-7.

特异性

Clone 5C1.2 targets an internal epitope present in human spliced isoforms 1&2 (SEP3A and SEP3B), both mouse and all three rat spliced isoforms (G-septin-alpha, -beta, and -gamma) reporeted by UniProt (Q9UH03, Q9Z1S5, and Q9WU34, respectively). Target epitope is absent from human spliced isoform 3 (SEP3C).

免疫原

KLH-conjugatged linear peptide coresponding to an internal sequence from the N-terminal half of human septin-3.

应用

Western Blotting Analysis: A 1:1,000 dilution of from a representative lot detected Septin-3 in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Septin-3 in human cerebellum and cerebral cortex, as well as in mouse cerebral cortex tissue sections.

Research Category
Neuroscience
Anti-Septin-3, clone 5C1.2, Cat. No. MABN1153, is a highly specific mouse monoclonal antibody that targets Septin-3, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

质量

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Septin-3 in 10 µg of mouse brain tissue lysate.

目标描述

~40/39 kDa observed. 40.70/40.10 kDa (human isoform 1/2), 40.04/38.71 kDa (mouse isoform 1/2), and 40.60/39.62/34.30 kDa (rat isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified.
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆5C1.2, monoclonal
species reactivitymouse, human
species reactivity (predicted by homology)rat (based on 100% sequence homology)
technique(s)immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_663786
UniProt登记号Q9UH03
运输ambient
Gene Informationhuman ... SEPT3(55964)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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