Anti-SK2 (KCa2.2) Antibody, clone K78/29

产品说明

一般描述

Small conductance calcium-activated potassium channel protein 2 (UniProt P70604; also known as SK2, SKCa 2, SKCa2, KCa2.2) is encoded by the Kcnn2 gene (Gene ID 54262) in rat species. Small conductance calcium-activated potassium channels mediate cell membrane repolarization following intracellular Ca2+ increase induced by various stimuli, thereby restoring an excited cell back to its resting state. SK2 is involved in afterhyperpolarization (AHP) that follows action potentials in vertebrate neurons. SK2 regulates neuronal excitability by contributing to the slow component of synaptic AHP. Ca2+ activates SK2 through the Ca2+ sensor calmodulin (CaM), which can bind up to four Ca2+ ions. The dynamic coupling between Ca2+ binding to CaM, CaM binding to SK, and the open probability of SK are all essential components of the SK2 activity.

特异性

Clone K78/29 detects endogenous KCa2.2 in rat brain membrane extracts, as well as the transiently expressed rat SK2 (KCa2.2), but not SK1 (KCa2.1), SK3 (KCa2.3), SK4 (KCa3.1, KCa4), or Kv2.1 constructs in transfected COS-7 cells. Exprected to react with both spliced isoforms of human KCa2.2 reported by UniProt (Q9H2S1).

免疫原

Epitope: C-terminal cytomplasmic region.
Linear peptide corresponding to a C-terminal cytoplasmic domain sequence of rat SK2 (KCa2.2).

应用

Anti-SK2 (KCa2.2) Antibody, clone K78/29 is an antibody against SK2 for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected SK2 (KCa2.2) immunoreactivity in human cerebellum, human cerebral cortex, mouse brain, mouse skeletal muscle, rat kidney and rat testis tissue sections.
Western Blotting Analysis: A representative lot detected the transiently expressed rat SK2 (KCa2.2), but not SK1 (KCa2.1), SK3 (KCa2.3), SK4 (KCa3.1, KCa4), or Kv2.1 constructs in transfected COS-7 cells (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).
Immunohistochemistry Analysis: A representative lot detected SK2 (KCa2.2) immunoreactivity in adult rat brain hippocampus sections (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

质量

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected SK2 (KCa2.2) in 10 µg rat brain tissue lysate.

目标描述

~45 kDa observed. 63.85 kDa (rat), 63.76 kDa (human isoform 1), 26.34 kDa (human isoform 2), 91.60 kDa (mouse) calculated.

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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