产品说明
特异性
Recognizes type I alpha subunit of VGSC. Does not cross react with any other sodium channel antigens tested so far.
SPECIES REACTIVITIES: It is expected that the antibody may also react with human due to sequence homology. Other species have not been tested.
免疫原
Purified peptide from 465-481 of alpha subunit of rat type I voltage-gated sodium channel (VGSC) (Accession P04774).
应用
This Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I is validated for use in IH, WB for the detection of Sodium Channel.
All procedures that are going to receive a full-length protein should be performed at 4C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).
Western blot: 1:200 using ECL on rat brain membranes.
Immunohistochemistry on rat brain fixed frozen sections and mouse heart tissue.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | mouse, rat |
manufacturer/tradename | Chemicon® |
technique(s) | immunohistochemistry: suitable western blot: suitable |
NCBI登记号 | NM_006920.4 |
UniProt登记号 | P35498 |
运输 | dry ice |
Gene Information | human ... SCN1A(6323) |
安全信息
危险声明 | H412 |
预防措施声明 | P273 - P501 |
危险分类 | Aquatic Chronic 3 |
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
Sigma-Aldrich