产品说明
特异性
Recognizes vimentin in cultured mammalian cells and tissue sections. AB1620 specifically stains the vimentin band (58 kD) using total cell or tissue extracts in immunoblotting. No reactivity to desmin, keratin, neurofilaments or glial filaments. Shows wide cross-reactivity among mammalian species using various immunolabeling techniques.
免疫原
Vimentin purified from cultured human foreskin fibroblasts.
应用
Research Category
Cell Structure
Anti-Vimentin Antibody detects level of Vimentin & has been published & validated for use in IC, IH, IH(P) & WB.
Immunohistochemistry: 1:20-1:40. Shows reactivity on frozen tissue sections as well as formalin-fixed paraffin embedded tissue by immunoperoxidase and immunofluorescent assays.
Immunocytochemistry
Western blot
Optimal working dilutions must be determined by end user.
Research Sub Category
Cytoskeleton
目标描述
58 kDa
外形
Unpurified
Neat goat Serum. Liquid containing 0.1% sodium azide.
储存及稳定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Breast carcinoma tissue, Colon, Prostate, Tonsil, Tonsilar lymphoma, Uterus, Ureter
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | goat |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human |
species reactivity (predicted by homology) | mammals |
manufacturer/tradename | Chemicon® |
technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
NCBI登记号 | NM_003380.2 |
UniProt登记号 | P08670 |
运输 | dry ice |
Gene Information | human ... VIM(7431) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich