产品说明
应用
APN-Azide is a bifunctional crosslinker for thiol-to-alkyne coupling. This coupling can be performed with high selectivity in biological medium using mild reaction conditions.
Standard protein labeling procedure (cysteine labeling)
1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
3. Incubate at room temperature for 2 hours.
4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.
5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.
*Note: avoid thiol-containing buffers.
包装
50 mg in glass bottle
基本信息
| 经验(实验)分子式 | C9H4N4 |
| 分子量 | 168.15 |
| PubChem化学物质编号 | 329769021 |
产品性质
| 质量水平 | 100 |
| 测定 | 95% |
| 形式 | powder |
| reaction suitability | reagent type: cross-linking reagent |
| mp | 100 ℃ |
| 储存温度 | 2-8℃ |
| SMILES string | N#CC#CC1=CC=C(N=[N+]=[N-])C=C1 |
| InChI | 1S/C9H4N4/c10-7-1-2-8-3-5-9(6-4-8)12-13-11/h3-6H |
| InChI key | BDLXITYEYYHQNK-UHFFFAOYSA-N |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H228 - H315 - H319 - H335 |
| 预防措施声明 | P210 - P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 4.1B - Flammable solid hazardous materials |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
