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APN-Maleimide

品牌
Sigma-Aldrich
货号
806536
包装型号
规格纯度
参考价格
1055.87 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
APN-Maleimide
3-(4-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)phenyl)propiolonitrile
MAPN
产品介绍:

产品说明

应用

APN-Maleimide is a bifunctional crosslinker for thiol-to-thiol coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. Due to kinetic resolution the first thiol reacts exclusively with the maleimide residue producing the protein-APN conjugate. This conjugate can be readily coupled with thiol-containing molecules.

包装

50 mg in glass bottle

制备说明

Standard protein labeling procedure (cysteine labeling).

  • Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
  • Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
  • Incubate at room temperature for 10 minutes.
  • If necessary, purify the protein-APN conjugate using size exclusion chromatography or ultrafiltration.
  • The conjugate can be readily coupled with thiol-containing substrates by incubating the components in aqueous buffer (pH 6.5-9.0) at ambient temperature for 2 hours.
*Note: avoid thiol-containing buffers.

基本信息

经验(实验)分子式C13H6N2O2
分子量222.20
PubChem化学物质编号329769017
NACRESNA.22

产品性质

形式powder
质量水平100
储存温度2-8℃
SMILES stringN#CC#CC1=CC=C(N2C(C=CC2=O)=O)C=C1
InChI1S/C13H6N2O2/c14-9-1-2-10-3-5-11(6-4-10)15-12(16)7-8-13(15)17/h3-8H
InChI keyCHKKXKRQICWZFF-UHFFFAOYSA-N

安全信息

象形图GHS07
警示用语:Warning
危险声明H302 - H315 - H319 - H335
预防措施声明P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338
危险分类Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官Respiratory system
储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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