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AttoPhos

品牌
Roche
货号
11681982001
规格纯度
sufficient for 1,800 multiwell tests (100 ul each), set of (3 components), suitable for PCR
参考价格
7637.67 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
AttoPhos
产品介绍:

产品说明

一般描述

AttoPhos is a highly sensitive fluorimetric substrate for the detection of alkaline phosphatase. With an optimal excitation wavelength of 430–440 nm and the emission maximum at 560 nm, an exceptionally large Stokes Shift is seen. This results in an extremely low intrinsic background signal with a detection limit of 10–19 moles for alkaline phosphatase. In addition, the background fluorescence caused by biological molecules (assay components), cuvettes, or microplates is greatly reduced. With these unique properties, AttoPhos is more sensitive by a factor of 100 than the most commonly used fluorescence substrate 4-methylumbelliferyl-phosphate (MUP).

应用

AttoPhos has been used to detect bound probes by an image capture system.
It has also been used as a fluorescence substrate in the study to detect antigens by quantitative immuno-PCR (polymerase chain reaction).

包装

包含3个组分。

制备说明

Working concentration: AttoPhos Substrate
Optimal concentration 0.5–1.5 mM with alkaline phosphatase
Working solution: AttoPhos working solution
Transfer the entire content of one bottle AttoPhos buffer (bottle 2) into one bottle AttoPhos substrate (bottle 1).
The resulting AttoPhos substrate concentration is 1 mM.

Calibrator solution
Prepare appropriate dilutions of Calibrator stock solution (bottle 3) using AttoPhos buffer solution (bottle 2).
Storage conditions (working solution): AttoPhos working solution
Stable at 2 to 8 ℃ for 2 weeks.
When stored at -15 to -25 ℃, the solution is stable for at least 6 months.
Note: To avoid inhomogenity after thawing, mix thoroughly.Calibrator solution
Stable at 2 to 8 ℃ for at least 6 months.

其他说明

仅用于生命科学研究。不可用于诊断。

产品性质

用途 sufficient for 1,800 multiwell tests (100 ul each)
质量水平100
manufacturer/tradenameRoche
包装set of (3 components)
technique(s)PCR: suitable
颜色 fluorescent yellow
运输wet ice
储存温度2-8℃

组份列表

组份不可单独销售

货号组份
AttoPhos Substrate
AttoPhos Buffer
Calibration Solution

安全信息

象形图GHS05GHS07GHS08
警示用语:Danger
危险声明H302 + H332 - H315 - H318 - H373
预防措施声明P260 - P264 - P280 - P304 + P340 + P312 - P305 + P351 + P338 + P310 - P314
危险分类Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2
储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)does not flash
闪点(C)does not flash

Sigma-Aldrich

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