产品说明
一般描述
Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2. Also suitable for measuring the peptidase activity of the 20S proteasome. Typical concentrations range from 10 - 100 µM, depending on assay conditions.
Fluorogenic substrate for the quantitative determination of porcine calpain I and II activity. Also serves as a substrate for other proteases including proteasome. (excitation wavelength: 380 nm; emission wavelength: 460 nm)
包装
25 mg in Plastic ampoule
生化/生理作用
Product does not compete with ATP.
Reversible: no
Primary Target
Calpain -1 and -2
Cell permeable: no
警告
Toxicity: Standard Handling (A)
序列
Suc-Leu-Tyr-AMC
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
其他说明
Seol, J.H., et al. 1989. FEBS Lett. 247, 197.
Woo, K.M., et al. 1989. J. Biol. Chem. 264, 2088.
Sasaki, T., et al. 1984. J. Biol. Chem.259, 12489.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C29H31N3O7 |
| 分子量 | 533.57 |
| MDL编号 | MFCD00057900 |
产品性质
| 质量水平 | 100 |
| 测定 | ≥95% (TLC) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze desiccated protect from light |
| 颜色 | off-white |
| 溶解性 | DMSO: soluble |
| 荧光 | λex ~380 nm λem ~460 nm |
| 运输 | ambient |
| 储存温度 | −20℃ |
| InChI | 1S/C29H33N3O8/c1-4-16(2)27(32-24(34)11-12-25(35)36)29(39)31-22(14-18-5-8-20(33)9-6-18)28(38)30-19-7-10-21-17(3)13-26(37)40-23(21)15-19/h5-10,13,15-16,22,27,33H,4,11-12,14H2,1-3H3,(H,30,38)(H,31,39)(H,32,34)(H,35,36)/t16?,22?,27-/m0/s1 |
| InChI key | VSWNAJAOJZXWBM-FTJGSZSVSA-N |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
