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ChIPAb+ HDAC2-ChIP Validated Antibody and Primer Set

品牌
Sigma-Aldrich
货号
17-10237
包装型号
规格纯度
from mouse
参考价格
6850.06 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
ChIPAb+ HDAC2-ChIP Validated Antibody and Primer Set
YY1-associated factor 1, histone deacetylase 2, transcriptional regulator homolog RPD3
产品介绍:

产品说明

一般描述

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ HDAC2 set includes the HDAC2 antibody, a Normal Mouse IgG, and control primers which amplify a 92 bp region of ChIP Primers, VWF promoter. The HDAC2 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HDAC2 -associated chromatin.

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

特异性

This antibody recognizes HDAC2 at the C-terminus.
Predicted to cross-react with rat based on sequence homology.

免疫原

KLH-conjugated, synthetic peptide (CEKTDTKGTKSEQLSNP) corresponding to human HDAC2 at the C-terminus.
Epitope: C-terminus

应用

Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from untreated or UV treated (6 hrs, 50 joules/m2.) U2OS cells (3 X 10E6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using using 2 µg of either Normal Mouse IgG or 2 µg of Anti-HDAC2 and the Magna ChIP A/G Kit (Cat. # 17-10085). Successful immunoprecipitation of HDAC2 associated DNA fragments was verified by qPCR using ChIP Primers, VWF promoter. Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. (Figure 2).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC2 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates HDAC2 (~60 kDa). (Figure 3).
Immunoprecipitation and HDAC Assay:
Representative lot data.
3 μL of a previous lot was used to immunoprecipitate HDAC activity from HeLa nuclear extract, which was then measured using the HDAC Assay Kit (Fluorometric Detection) (Catalog # 17-356) (Figure 4).



This ChIPAb+ HDAC2 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

包装

25 assays per set. Recommended use: ~2 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).

质量

Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HEK293T cells (5 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Mouse IgG, or 2 µg of Anti-HDAC2 and the Magna ChIP® A/G Kit (Cat. # 17-10085). Successful immunoprecipitation of HDAC2 associated DNA fragments was verified by qPCR using ChIP Primers, VWF promoter (Figure 1).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details

目标描述

~60 kDa

外形

Anti-HDAC2 (mouse monoclonal). One vial containing 50 µg of purified mouse monoclonal IgG in buffer containing 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20° C.
Concentration: 0.94 mg/mL
Normal Mouse IgG. One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers, VWF promoter. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human von Willebrand factor. Store at -20°C.
FOR: GCT GAG AGC ATG GCC TAG GGT GGT GGG CGG CAC
REV: CCC CTG CAA ATG AGG GCT GCG GCT ATC TCC AAG

Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

分析说明

Control
Includes normal mouse IgG and primers specific for human von Willebrand factor.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.32

产品性质

质量水平100
生物来源mouse
克隆monoclonal
species reactivityhamster, mouse, bovine, rat, human
manufacturer/tradenameChIPAb+
Upstate®
technique(s)ChIP: suitable
western blot: suitable
同位素/亚型IgG1
NCBI登记号NP_001518
UniProt登记号Q92769
运输dry ice
Gene Informationhuman ... HDAC2(3066)

安全信息

储存分类代码10 - Combustible liquids

Sigma-Aldrich

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