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ChIPAb+ Trimethyl-Histone H3 (Lys9)-ChIP Validated Antibody and Primer Set

品牌
Sigma-Aldrich
货号
17-10242
规格纯度
clone CMA308, from mouse
参考价格
6993.57 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
ChIPAb+ Trimethyl-Histone H3 (Lys9)-ChIP Validated Antibody and Primer Set
H3K9me3, Histone H3 (tri methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
产品介绍:

产品说明

一般描述

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Trimethyl-Histone H3 (Lys9) set includes the Trimethyl-Histone H3 (Lys9) antibody, a Normal mouse IgG, and control primers which amplify a 117 bp region of ChIP Primers, ZNF554. The Trimethyl-Histone H3 (Lys9) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Trimethyl-Histone H3 (Lys9) -associated chromatin.

特异性

This antibody recognizes Histone H3 trimethylated at Lys9. Some cross-reactivity with dimethyl Lys9 is detected at higher concentrations, but the difference in specificity is >27-fold1. Phosphorylation of Ser10 interferes with antibody binding to trimethyl Lys91.
Broad species cross-reactivity is expected, based on sequence homology.

免疫原

KLH-conjugated synthetic peptide corresponding to human Histone H3 trimethylated on Lys9.
Epitope: Trimethyl Lys9

应用

This ChIPAb+ Trimethyl-Histone H3 (Lys9) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µL of either Normal Mouse IgG,or 1 µL of Anti-Trimethyl Histone H3 (Lys9), and the Magna ChIP® G Kit (Cat. # 17-611). Successful immunoprecipitation of trimethyl Histone H3 (Lys9) associated DNA fragments was verified by qPCR using ChIP primers specific for the human ZNF554 region as a positive locus and human GAPDH primers as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP G (Cat. # 17-409) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
Recombinant Histone H3 (Cat. #14-494) (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-trimethyl Histone H3 (Lys9), clone CMA308 (2 μg/mL). Proteins were visualized using
a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates trimethyl Histone H3 (Lys9) (~17 kDa). (Figure 3).

包装

25 assays per set. Recommended use: ~1 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).

质量

Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µL of either Normal Mouse IgG, or 1 µL of Anti-Trimethyl Histone H3(Lys9), and the Magna ChIP® G Kit (Cat. # 17-611). Successful immunoprecipitation of trimethyl Histone H3 (Lys9) associated DNA fragments was verified by qPCR using ChIP primers specific for the human ZNF554 region as a positive locus(Figure 1). Please refer to the EZ-Magna ChIP G (Cat. # 17-409) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.

目标描述

~17 kDa

外形

Anti-Trimethyl-Histone H3 (Lys9) (mouse monoclonal). One vial containing 25 µL of purified mouse monoclonal IgG1 in PBS with 0.05% sodium azide before the addition of glycerol to 30%. Store at -20°C.
Normal Mouse IgG. One vial containing 25 µg of purified IgG in 25 µL of storage buffer containing 0.1% sodium azide. Store at -20°C.
Control Primers, ZNF554. One vial containing 75 μL of 5 μM of each primer specific for the 3’ end of human ZNF554. Store at -20°C.
FOR: CGG GGA AAA GCC CTA TAA AT
REV: TCC ACA TTC ACT GCA TTC GT

Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Includes normal mouse IgG and primers specific for human ZNF554.

法律信息

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
克隆CMA308, monoclonal
species reactivityvertebrates, human
manufacturer/tradenameChIPAb+
Upstate®
technique(s)ChIP: suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NM_003493
UniProt登记号Q16695
运输dry ice

安全信息

储存分类代码10 - Combustible liquids

Sigma-Aldrich

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