ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye

产品说明

一般描述

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Phycoerythrin (PE) is intensely bright, stable fluorescent dye that exhibits an excitation max at 566 nm and an emission max at 574 nm with an extinction coefficient of 1,960,000 cm^-1 M^-1. It is composed of αβ monomers that are organized in a disk-shaped trimer or hexamer. The α subunit of R-PE contains only the phycoerythrobilin (PEB) chromophore, while β and γ subunits contain both PEB and phycourobilin (PUB). PE has stronger fluorescence and high photostability. PE can be used for covalent labeling of antibodies and detection by flow cytometry.

应用

Flow Cytometry: Excitation: 566 nm, Emission: 574 nm

相容性

Requires ColorWheel^® Antibody (Sold Separately)

外形

CWDS-PE-25UL Set Components

CWD-PE-25UL - ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL - ColorWheel^® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

重悬

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质