产品说明
一般描述
Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 0.25 to 3.09 kbp
应用
DNA Molecular Weight Marker XVI (250 bp ladder) may be used as a molecular weight marker in agarose gel electrophoresis:
序列
The mixture contains 12 blunt-ended, double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 250, 500, 750, 1000, 1250, 1500, 1750, 2000, 2250, 2500, 2750, and 3000. The 1000- and 2000-bp banding pattern are 3 times brighter. Electrophoretic separation of this molecular weight marker results in a regular pattern.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The fragments exhibit uniform spacing (e.g., on a 0.7% agarose gel) for easier reading, while bands 1000 and 2000bp are 3 times brighter for easy orientation.
单位定义
50 μg = 1A260 unit
外形
Ready-to-use solution, 250 μg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).
制备说明
Storage conditions (working solution): 2 to 8 ℃
Once thawed, store at 2 to 8 ℃.
其他说明
仅用于生命科学研究。不可用于诊断。
产品性质
形式 | solution |
质量水平 | 100 |
包装 | pkg of 50 μg (in 200 μl) |
manufacturer/tradename | Roche |
运输 | dry ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | does not flash |
闪点(C) | does not flash |
Sigma-Aldrich