Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli

产品说明

一般描述

Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.
Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.

包装

50 μL in Plastic ampoule

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.

外形

Sterile-filtered in 20 mM Tris-HCl, pH 7.5.

重悬

There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.

其他说明

Murata, T., et al. 2003. Eur. J. Biochem.270, 3709.
Scudder, P., et al. 1986. Eur. J. Biochem.157, 365.
Scudder, P., et al. 1984. J. Biol. Chem.259, 6586.
Scudder, P., et al. 1983. Biochem. J.213, 485.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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