GDS MPX ID for Top STEC

产品说明

一般描述

Assurance^® GDS is an advanced genetic detection system for food pathogens. The MPX ID for Top STEC is a secondary automated nucleic acid amplification system for the identification of “Top Six” non-O157 Shiga Toxigenic E. coli (STEC). The Top 6 non-O157 STEC are defined as E. coli belonging to serogroups O103, O111, O121, O145, O26, or O45 that possess both the eae gene and at least one of the Shiga Toxin genes (stx1 or stx2). GDS MPX ID utilizes a proprietary PickPen^® IMS-based sample preparation procedure to quickly and effectively capture organisms belonging to the 6 specific Top STEC O-serogroups prior to genetic analysis for the associated pathogenicity genes. Each of the 3 unique eae gene sequences targeted by MPX ID are highly correlated plus one or more of the 6 O-serogroups comprising Top 6 non-O157 STEC. Because there are 3 different eae gene targets and 6 different O-serogroup targets, the PCR reactions have been divided between 2 Amplification Tubes. Each tube contains an internal control and all necessary PCR reagents. Results will automatically be combined between the 2 tubes via GDS Rotor-Gene^® software.

应用

Assurance^® GDS MPX ID for Top STEC is designed to follow GDS MPX Top 7 STEC (PN 71015BC) for the identification of “Top Six” non-O157 Shiga Toxigenic E. coli (STEC) in a variety of foods, ingredients, and environmental samples. Additionally, GDS MPX ID can be used to confirm isolated colonies from samples presumptive positive for Top 6 STEC.

特点和优势

• Designed specifically to follow GDS MPX Top STEC assays
• Identification of the specific O serogroup(s) responsible for the positive STEC result and verifies that all targets relevant to the definition of a Top 6 STEC (eae, stx and O group) are present within a single organism
• Confirm isolated colonies from samples presumptive positive for Top 6 STEC
• Utilizes the same single-step mEHEC^® enrichment media common to all BioControl methods for Salmonella, E.coli O157:H7, Shiga Toxin genes, and Top STEC
• Only 14 hours from start to finish

组分

• MPX Group 1 STEC Amplification Tubes
• MPX Group 2 STEC Amplification Tubes
• Top 6 STEC Concentration Reagent
• Resuspension Buffer Tq
• Top STEC Wash Solution

相容性

BioControl GDS

测试方法

PCR, Immunomagnetic Separation (IMS)

法律信息

AOAC is a registered trademark of AOAC International
Assurance is a registered trademark of BioControl Systems, Inc.
Assurance GDS is a registered trademark of BioControl Systems, Inc.
PICKPEN is a registered trademark of BioControl Systems, Inc.
Rotor-Gene is a registered trademark of Qiagen GmbH
mEHEC is a registered trademark of BioControl Systems, Inc.

产品性质

安全信息

象形图
警示用语：	Warning
危险声明	H290
预防措施声明	P234 - P390
危险分类	Met. Corr. 1
储存分类代码	8A - Combustible, corrosive hazardous materials
WGK	WGK 3