产品说明
一般描述
KAPA PROBE FAST qPCR Kits 为所有基于探针的 qPCR 应用提供快速且可重复的结果。试剂盒包含即用型预混液,可使用序列特异性荧光探针化学,包括水解探针(例如,TaqMan ®)、FRET 探针和置换探针(例如,分子信标),实现高灵敏度和高准确度的实时 PCR。
应用
KAPA PROBE FAST可用于:
特点和优势
准确区分等位基因:
质量
KAPA PROBE FAST qPCR Master Mix (2X) 经过严格的质量控制检测,不含污染的外切和核酸内切酶活性,并且符合 DNA 污染水平方面的严格要求。
制备说明
ROX/荧光素引用染料(取决于试剂盒配置)对光敏感。长时间暴露于直射光下会导致荧光信号强度的损失。避免反复冻融。使用前务必确保产品已完全解冻并混合。
KAPA PROBE FAST qPCR Master Mix (2X) 可在 30 次冻融循环中保持稳定。确保所有试剂在不使用时均避光储存于-20℃ 条件下。避光条件下,试剂在 4℃ 避光条件下可稳定保存至少一周,只要不被微生物和/或核酸酶污染,可在此温度下短期使用。
其他说明
仅用于研究目的。不用于诊断程序。
法律信息
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
QuantStudio is a trademark of IROA Technologies LLC
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
基本信息
NACRES | NA.55 |
产品性质
质量水平 | 100 |
用途 | sufficient for 100 reactions sufficient for 1000 reactions sufficient for 500 reactions sufficient for 5000 reactions |
保质期 | ≤12 mo. |
特点 | dNTPs included hotstart |
包装 | kit of 1 mL (100 x 20 μL rxn; KK4701) kit of 10 mL (1000 x 20 μL rxn; KK4703) kit of 5 mL (500 x 20 μL rxn; KK4702) kit of 50 mL (5000 x 20 μL rxn; KK4715) |
manufacturer/tradename | Roche |
浓度 | 2 × |
technique(s) | qPCR: suitable |
input | purified DNA |
相容性 | for use with Agilent Mx3000P for use with Agilent Mx3005P for use with Agilent Mx4000 for use with Applied Biosystems® 5700 for use with Applied Biosystems® 7000 for use with Applied Biosystems® 7300 for use with Applied Biosystems® 7500 for use with Applied Biosystems® 7700 for use with Applied Biosystems® 7900HT for use with Applied Biosystems® ViiA 7 for use with QuantStudio™ for use with StepOne™ for use with StepOnePlus™ |
detection method | probe-based |
运输 | wet ice |
储存温度 | −20℃ |
组份列表
组份不可单独销售
货号 | 组份 |
KAPA Taq HotStart® DNA Polymerase | |
50X ROX™ High Reference Dye | |
50X ROX™ Low Reference Dye | |
reaction buffer | |
dNTPs | |
MgCl2 5 mM |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | does not flash |
闪点(C) | does not flash |