产品说明

一般描述

KAPA Taq HotStart^® with dNTPs is supplied in a 2X ReadyMix^™ format containing all the components required for PCR except primers and template-simply use PCR-grade water to make up the required reaction volume. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye. KAPA Taq DNA Polymerase is the single-subunit Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli.

应用

KAPA Taq HotStart^® has been used for:

• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR
• Aquarium DNA extraction to generate marker gene libraries

生化/生理作用

KAPA Taq HotStart^® DNA polymerase and KAPA Taq exhibit 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The enzyme shows an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity. KAPA Taq hotstart buffer is formulated uniquely to facilitate specific primer annealing. This translates to higher yields of the specific product when compared to traditional Taq buffers. It also enhances the amplification of GC- and AT-rich templates. KAPA Taq hotstart DNA polymerase might be used in combination with any standard Taq buffer with a pH of 8.3 or higher

特点和优势

High performance

• Improved sensitivity, specificity, and yields.
• Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.

• KAPA Taq HotStart^® DNA Polymerase can replace any commercial hotstart Taq DNA polymerase in an existing protocol. The final MgCl₂ concentration and annealing temperature may need to be optimized to account for differences in formulation.
• The KAPA Taq HotStart Buffer is a uniquelyformulated buffer offering improved specificity and sensitivity, and improved amplification of GC- and AT-rich templates.
• The KAPA Taq HotStart Buffer does not contain MgCl₂; MgCl₂ (25 mM) is supplied separately to allow greater flexibility during reaction setup.
• The KAPA Taq HotStart PCR Kit is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

质量

Each batch of KAPA Taq HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq HotStart PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

制备说明

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4℃ for short-term use (up to 1 month). Return to -20℃ for long term storage.

其他说明

For Research Use Only. Not for use in diagnostic procedures.

法律信息

HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC

基本信息

产品性质

组份列表

组份不可单独销售

安全信息

象形图
警示用语：	Warning
危险声明	H302 - H371
预防措施声明	P260 - P264 - P270 - P301 + P312 + P330 - P308 + P311 - P501
危险分类	Acute Tox. 4 Oral - STOT SE 2
储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	does not flash
闪点(C)	does not flash