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MISSION® TRC3 ORF BFP DNA Control

品牌
Sigma-Aldrich
货号
ORFBFP
包装型号
规格纯度
参考价格
3909.8 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
MISSION® TRC3 ORF BFP DNA Control
产品介绍:

产品说明

一般描述

The MISSION TRC3 ORF BFP Control Vector is a lentivirus plasmid vector. The vector contains an ORF insert expressing BFP, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole Genome LentiORF Pool.

This positive control can be used for measuring transduction efficiency and optimizing ORF delivery in cell lines that do not survive any puromycin exposure. The BFP Control contains a gene encoding BFP driven by the EF1a promoter. This control provides fast visual confirmation of successful transduction. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively.

In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The MISSION TRC3 ORF BFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

应用

Functional Genomics/Overexpression Screening/Target Validation

法律信息

Use of this product is subject to one or more license agreements. For details, please see the MISSION License Page
MISSION is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.51

产品性质

包装vial of 20 μL
质量水平200
浓度500 ng/μL
application(s)genome mapping
genomic analysis
运输dry ice
储存温度−70℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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