产品说明
一般描述
Nitrilase belongs to the C-N hydrolase superfamily. It possesses the catalytic triad motifs: glutamate, lysine, and cysteine and are distributed in fungi, plants, and animals.The isoelectric point (pI) of this protein is 8.1. Native polyacrylamide gel electrophoresis (PAGE).
应用
Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies.
生化/生理作用
Nitrilases catalyze the liberation of ammonia and the formation of carboxylic acid from the nitriles. The oligomeric structural organization of nitrilase is crucial for its functionality. It displays broad substrate specificity.
包装
Bottomless glass bottle. Contents are inside inserted fused cone.
单位定义
1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25℃ with the conversion of acrylonitrile to acrylic acid
基本信息
酶学委员会(EC)编号 | 3.5.5.1 ( BRENDA | IUBMB ) |
MDL编号 | MFCD04040969 |
NACRES | NA.54 |
产品性质
质量水平 | 100 |
重组 | expressed in E. coli |
形式 | crystals powder |
specific activity | ≥2.0 U/mg |
分子量 | 41 kDa |
储存温度 | 2-8℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H317 - H334 |
预防措施声明 | P261 - P280 - P342 + P311 |
危险分类 | Resp. Sens. 1 - Skin Sens. 1 |
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich