产品说明
一般描述
Papanicolaou′s solution 3a polychromatic solution EA 31 - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou′s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Product number 1.05175), Papanicolaou′s solution 1a Harris hematoxylin solution, (Product number 1.09253) or Papanicolaou′s solution 1b Hematoxylin solution S, (Product number 1.09254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution (Papanicolaou′s solution 2b Orange II solution, Product number 1.106887 or Papanicolaou′s solution 2a Orange G solution (OG6), Product number 1.06888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, (Papanicolaou′s solution 3a polychromatic solution EA 31, (Product number 1.09271), Papanicolaou′s solution 3b polychromatic solution EA 50, (Product number 1.09272), Papanicolaou′s solution 3c polychromatic solution EA 65 (Product number 1.09270), o Papanicolaou′s solution 3d polychromatic solution EA 65, (Product number 1.09269). The polychrome solution is used for demonstration of differentiation of squamous cells. Papanicolaou′s solution 3a polychromatic solution EA 31 gives a blue-green to green staining result with clinical material.
The 500 ml bottle provides 1500 - 2000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
应用
Used to demonstrate the differentiation of squamous cells in gynecological cytology
分析说明
Suitability for microscopy (Vaginal smear):passes test
Nuclei:blue to dark violet
Cyanophilic cytoplasm:blue-green to green
Eosinophilic cytoplasm:pink
产品性质
质量水平 | 400 |
pH值(酸碱度) | 5.0-5.5 (20 ℃ in H2O) |
转变温度 | flash point 17 ℃ |
密度 | 0.82 g/cm3 at 20 ℃ |
储存温度 | 20-25℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H225 - H302 + H312 + H332 - H319 - H370 |
预防措施声明 | P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P308 + P311 |
危险分类 | Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 1 |
储存分类代码 | 3 - Flammable liquids |
WGK | WGK 2 |
闪点(F) | 62.6 °F |
闪点(C) | 17 ℃ |
Sigma-Aldrich