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PCR DIG Labeling MixPLUS

品牌
Roche
货号
11835289910
规格纯度
sufficient for 2 x 50 assays (50μl final reaction volume), pkg of 2 × 250 μL, solution
参考价格
6587.9 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
PCR DIG Labeling MixPLUS
产品介绍:

产品说明

一般描述

The PCR DIG Labeling MixPLUS is a mixture of lithium salts of deoxyadenosine triphosphate (dATP), deoxycytosine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP), deoxyuridine triphosphate (dUTP) and digoxigenin (DIG)-11-dUTP. This nucleotide mixture can be added directly to polymerase chain reactions (PCR) and the DIG-labeled nucleotide will be incorporated into the PCR product. The incorporation of dUTP instead of deoxythymidine triphosphate (dTTP) allows the degradation of contaminating PCR products from previous amplification reactions with uracil-DNA glycosylase (UNG), preventing carryover contamination.The incorporated DIG label renders the sensitive detection of the PCR product with anti-DIG-conjugates, for example in the PCR ELISA, DIG-labling, and PCR ELISA, DIG-Detection. The PCR DIG Labeling MixPLUS gives comparable signal intensities to the PCR DIG Labeling Mix.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

应用

PCR DIG Labeling MixPLUS is used for direct labeling of amplification products with digoxigenin (DIG)-deoxyuridine triphosphate (dUTP) in polymerase chain reactions (PCR) and for carryover prevention.The PCR DIG Labeling Mixplus is 10x concentrated. For each reaction, add 10% of the final volume to the reaction mix.

质量

The PCR DIG Labeling MixPLUS is function tested.

其他说明

仅用于生命科学研究。不可用于诊断。

产品性质

形式solution
质量水平100
用途 sufficient for 2 x 50 assays (50μl final reaction volume)
包装pkg of 2 × 250 μL
manufacturer/tradenameRoche
环保替代产品特性Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
环保替代产品分类Aligned
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)does not flash
闪点(C)does not flash

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