PhosphoDetect Anti-Smad3 (pSer^423/425) Rabbit mAb (C25A9)

产品说明

一般描述

Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser^423/425 in HT-1080 cells treated with TGF-β1.
Purified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser^423/425.
This PhosphoDetect Anti-Smad3 (pSer^423/425) Rabbit mAb (C25A9) is validated for use in Immunoblotting, Immunoprecipitation for the detection of Smad3 (pSer^423/425).

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser ^423/425 phosphorylation sites of Smad3, coupled to KLH.

包装

50 μL in Plastic ampoule

应用

Immunoblotting (1:1000)
Immunoprecipitation (1:50)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5.

其他说明

Attisano, L., et al. 2002. Science 296, 1646.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.
Does not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween^®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine, xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

产品性质

安全信息