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PhosphoDetect Anti-Syk (pTyr525/526) Rabbit pAb

品牌
Sigma-Aldrich
货号
PK1010
规格纯度
liquid, Calbiochem®
参考价格
4848.93 *本价格含增值税费
促销
服务
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数量
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产品名称:
PhosphoDetect Anti-Syk (pTyr525/526) Rabbit pAb
产品介绍:

产品说明

一般描述

This PhosphoDetect Anti-Syk (pTyr525/526) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of Syk (pTyr525/526).
Recognizes the ~72 kDa Syk protein phosphorylated at Tyr525/526 in human Syk or Tyr519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.
Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~72 kDa Syk protein phosphorylated on Tyr525/526.

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526 phosphorylation sites of human Syk
Human

包装

50 μL in Plastic ampoule

应用


ELISA (1:1000)
Immunoblotting (1:1000)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

重悬

Do not aliquot.

分析说明

Positive Control
293T cells transfected with wild-type Syk and CD8

其他说明

Coopman, P.J., et al. 2000. Nature406, 742.
Turner, M., et al. 2000. Immunol. Today21, 148.
Zang, J., et al. 2000. J. Biol. Chem.275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev.165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol.9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol.9, 309.
Tyr525/526 represent the phosphorylation sites in human Syk, while Tyr519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式liquid
不包含 preservative
species reactivitymouse, human
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
同位素/亚型IgG
运输wet ice
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

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