PhosphoDetect Anti-Syk (pTyr^525/526) Rabbit pAb

产品说明

一般描述

This PhosphoDetect Anti-Syk (pTyr^525/526) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of Syk (pTyr^525/526).
Recognizes the ~72 kDa Syk protein phosphorylated at Tyr^525/526 in human Syk or Tyr^519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.
Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~72 kDa Syk protein phosphorylated on Tyr^525/526.

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^525/526 phosphorylation sites of human Syk
Human

包装

50 μL in Plastic ampoule

应用

ELISA (1:1000)
Immunoblotting (1:1000)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

重悬

Do not aliquot.

分析说明

Positive Control
293T cells transfected with wild-type Syk and CD8

其他说明

Coopman, P.J., et al. 2000. Nature406, 742.
Turner, M., et al. 2000. Immunol. Today21, 148.
Zang, J., et al. 2000. J. Biol. Chem.275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev.165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol.9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol.9, 309.
Tyr^525/526 represent the phosphorylation sites in human Syk, while Tyr^519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween^®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

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