Sulfatase from Aerobacter aerogenes

产品说明

一般描述

Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.

应用

Sulfatase from Aerobacter aerogenes has been used:

• as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
• in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
• to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides

生化/生理作用

Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.

单位定义

One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 ℃.

外形

Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.

基本信息

产品性质

安全信息