产品说明
一般描述
Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.
产品规格
Clean in place with 0.1 M NaOH.
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法律信息
ToyoScreen is a registered trademark of Tosoh Corporation
Toyopearl is a registered trademark of Tosoh Corporation
产品性质
| 物料 | aluminum seal polypropylene and polyethylene frit |
| 产品线 | ToyoScreen® |
| specific analyte(s) | IgG |
| 包装 | pkg of 5 × 1 mL |
| manufacturer/tradename | Tosoh 23430 |
| 参数 | 0.2-1 mL/min flow rate 3 bar max. pressure 4 mL/min max. flow rate |
| technique(s) | HPLC: suitable |
| 长度 × 内径 | 3 cm × 6.4 mm |
| 基质 | hydroxylated methacrylic polymer |
| 基质活性基团 | rProtein A phase |
| 粒径 | 45 μm |
| 操作pH | 2 - 13 |
| 容量 | >70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time) >80 g/L adsorption capacity (IgG)(static) |
| 分离技术 | affinity |
Sigma-Aldrich
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