您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > TSKgel® Chelate-5PW HPLC Column

TSKgel® Chelate-5PW HPLC Column

货号
808645
规格纯度
phase iminodiacetic acid, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
参考价格
27914.4 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
TSKgel® Chelate-5PW HPLC Column
TSKgel Chelate-5PW HPLC Column and Guardgel Kit
Chelate-5PW
产品介绍:

产品说明

一般描述

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.

Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.

推荐产品

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

法律信息

TSKgel is a registered trademark of Tosoh Corporation

基本信息

eCl@ss32110501

产品性质

物料stainless steel
产品线TSKgel®
manufacturer/tradenameTosoh Chelate-5PW, 08645
标记范围20 μmol/mL resin ligand density
参数≤20% organic solvent
≤3 M salt concentration
≤45 ℃ temperature
1.2 mL/min flow rate
10 bar max. pressure
technique(s)HPLC: suitable
长度 × 内径7.5 cm × 7.5 mm
基质 polymer particle platform
基质活性基团iminodiacetic acid phase
粒径10 μm
孔径1000 Å
操作pH2 - 12
相容性technique used for affinity HPLC
分离技术affinity

Sigma-Aldrich

推荐产品