产品说明
一般描述
A cell-permeable, reversible, and stearated, 13-amino acid peptide corresponding to the N-terminus of MAPK/ERK kinase (MEK). Acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of ELK1. Selectively binds to ERK2 and prevents its interaction with MEK (IC50 = 2.5 µM). Shown to block ERK activity in PMA-stimulated NIH3T3 cells and NGF-treated PC12 cells (IC50 = 13 µM). Does not affect the activation of either JNKs or p38 MAP kinase.
A cell-permeable and reversible stearated 13-amino acid peptide corresponding to the N-terminus of MEK1 (MAPKK) that acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of Elk1. Selectively binds to ERK2 and prevents its interaction with MEK (IC50 = 2.5 µM). Potently blocks ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells (IC50 = 13 µM). Does not affect the activation of JNKs or p38.
包装
1 mg in Plastic ampoule
Packaged under inert gas
生化/生理作用
Primary Target
MEK
Product does not compete with ATP.
Reversible: yes
Cell permeable: yes
警告
Toxicity: Standard Handling (A)
序列
Ste-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH₂ (Ste = stearated)
外形
Supplied as a trifluoroacetate salt.
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
其他说明
Kelemen, B.R., et al. 2002. J. Biol. Chem.277, 8741.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C86H153N19O17S |
分子量 | 1757.32 |
产品性质
质量水平 | 100 |
测定 | ≥95% (HPLC) |
形式 | lyophilized solid |
效能 | 2.5 μM IC50 |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze desiccated (hygroscopic) protect from light |
颜色 | white |
溶解性 | water: 1 mg/mL DMSO: 5 mg/mL |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich