ATP Assay Kit

产品名称：

ATP Assay Kit
Luciferin-Related Kit

产品介绍：

产品说明

一般描述

Cell death (especially apoptosis) is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions, the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore, loss of ATP level in the cell has been used as an indicator of cell death. In contrast, increased levels of ATP has been an indicator of cell proliferation. Calbiochem^® brand ATP Assay Kit utilizes bioluminescent detection of the ATP levels for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter.
The assay is based on the firefly luciferase-catalyzed oxidation of D-luciferin in the presence of ATP and oxygen, whereby the amount of ATP is quantified by the amount of light (hv) produced.

包装

1 kit in Glass bottle

组分

One vial of Luciferase ATP Monitoring Enzyme, Enzyme Reconstitution Buffer, 1 bottle of Nucleotide Releasing Reagent, one vial of ATP Standard, and a user protocol.

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

产品规格

Assay Time: 1 h

原理

The assay can be fully automatic for high throughput (10 s/sample) and is extremely sensitive (detects 10-100 mammalian cells/well). The high sensitivity of this assay has led to many other applications for detecting ATP production in various enzymatic reactions, as well as for detecting low level bacterial contamination in samples such as blood, milk, urine, soil, and sludge.

制备说明

Reagent Reconstitution1. Reconstitute ATP Monitoring Enzyme with 2ml of the Enzyme Reconstitution Buffer. Mix well by gentle pipetting. The reconstituted enzyme is stable for up to 2 months at 4°C.2. Prepare an ATP standard solution by dissolving the 1 mg ATP into 1 ml of H₂O. The solution is stable for several weeks at -20°C.

储存及稳定性

Upon arrival store the entire contents of the kit at -70°C.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

用途	sufficient for 200 tests
质量水平	100
manufacturer/tradename	Calbiochem^®
储存条件	OK to freeze avoid repeated freeze/thaw cycles
input	sample type sludge sample type blood sample type milk sample type soil sample type urine
detection method	chemiluminescent
储存温度	−70℃

安全信息

象形图
警示用语：	Danger
危险声明	H302 - H315 - H318 - H373 - H410
预防措施声明	P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P314
危险分类	Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2 Oral
靶器官	Kidney
储存分类代码	10 - Combustible liquids
WGK	WGK 3